Journal: PLOS One

Article Title: Down-regulation of circ0001361 induces apoptosis and suppresses the progression of glioma

doi: 10.1371/journal.pone.0343681

Figure Lengend Snippet: (A) MiRNAs targeted by circ0001361 were predicted. (B) Expression of hsa-miR-525-5p in glioma (Group A) and normal brain tissue (Group B) was shown. (C) Expression of hsa-miR-525-5p in glioma grade Ⅱ -Ⅳ was shown. (D) Prognostic significance of hsa-miR-525-5p was analyzed. (E) Potential binding sites between circ0001361 and hsa-miR-525-5p were predicted. (F) Circ0001361-hsa-miR-525-5p interaction was confirmed by dual-luciferase reporter assay.

Article Snippet: After 48 hours, the Firefly luciferase and Renilla Luciferase were detected using the Dual Luciferase Reporter Assay Kit (Vazyme, China).

Techniques: Expressing, Binding Assay, Luciferase, Reporter Assay

Journal: PLOS One

Article Title: Down-regulation of circ0001361 induces apoptosis and suppresses the progression of glioma

doi: 10.1371/journal.pone.0343681

Figure Lengend Snippet: (A) Genes targeted by hsa-miR-525-5p were predicted. (B) Correlation between hsa-miR-525-5p and MEIS1 was analyzed. (C) Prognostic significance of MEIS1 was analyzed. (D) Expression of MEIS1 in GBM, LGG and normal brain tissues was shown. (E) Expression of MEIS1 in glioma grade Ⅱ -Ⅳ was shown. (F) The relationship between MEIS1 and IDH mutation status was shown. (G) The relationship between MEIS1 and 1p/19q co−deletion status was shown. (H) Potential binding sites between hsa-miR-525-5p and MEIS1 were predicted. (I) Hsa-miR-525-5p and MEIS1 interaction was confirmed by dual-luciferase reporter assay.

Article Snippet: After 48 hours, the Firefly luciferase and Renilla Luciferase were detected using the Dual Luciferase Reporter Assay Kit (Vazyme, China).

Techniques: Expressing, Mutagenesis, Binding Assay, Luciferase, Reporter Assay

Journal: The Kaohsiung Journal of Medical Sciences

Article Title: Modulation of RASD2 by miRNA ‐485‐5p Drives Thyroid Cancer Progression and Metastasis

doi: 10.1002/kjm2.70028

Figure Lengend Snippet: miR‐485‐5p regulation of RASD2 in thyroid cancer. (A) Venn diagram showing overlapping miRNA predictions from three databases (TargetScan, miRDB, StarBase). (B) Sequence alignment between miR‐485‐5p and RASD2 3′‐UTR showing predicted binding site. (C) Dual‐luciferase reporter assay results in IHH4 and TPC‐1 cells transfected with wild‐type or mutant RASD2 3′‐UTR constructs and miR‐485‐5p mimic or negative control. (D) Western blot analysis of RASD2 protein levels in cells transfected with miR‐485‐5p mimic or negative control. (E) TCGA database analysis of miR‐485‐5p expression in thyroid cancer vs. normal tissues. (F) miR‐485‐5p expression in paired tumor‐normal tissues by qRT‐PCR ( n = 96 pairs). (G) Correlation analysis between miR‐485‐5p and RASD2 expression levels in thyroid cancer tissues. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: After 48 h, luciferase activities were measured using the Dual‐Luciferase Reporter Assay System (HY‐K1013, MedChemExpress).

Techniques: Sequencing, Binding Assay, Luciferase, Reporter Assay, Transfection, Mutagenesis, Construct, Negative Control, Western Blot, Expressing, Quantitative RT-PCR

Journal: bioRxiv

Article Title: A multivalent peptide-polymer conjugate material mimics STING to therapeutically activate innate immune signaling

doi: 10.64898/2026.03.24.712780

Figure Lengend Snippet: ( A ) Sequence of the murine STING C-terminal tail peptide mSTING(340-378), highlighting motifs known to interact with TBK1 and IRF3. ( B ) Peptide-polyanion conjugate was synthesized through copper-catalyzed azide-alkyne cycloaddition of an azidolysine-mSTING(340-378) peptide and the polyanion poly(L-glutamate)-graft-alkyne. ( C ) HPLC chromatogram using size exclusion column of azidolysine-mSTING(340-378), poly(L-glutamate)-graft-alkyne, and poly(L-glutamate)-graft-mSTING(340-378). ( D ) Circular dichroism spectrum of azidolysine-mSTING(340-378), poly(L-glutamate)-graft-alkyne, and poly(L-glutamate)-graft-mSTING(340-378) in 1× PBS. ( E ) Activity of the peptide-polyanion conjugate was examined using a HEK293T-derived reporter with luciferase expression under the control of target transcription factor IRF3. IRF3 reporter signal relative to buffer treatment for HEK293T reporter cells treated with 2 μM STING or Scr peptide alone, conjugated to only C-terminus of size-matched poly(L-glutamate) to generate a monovalent peptide-polyanion conjugate control, or conjugated to side chains as specified in ( B ) to generate a multivalent peptide-polyanion conjugate. All treatments were transfected using TransIT-X2, activity was measured 24 h post treatment (N = 3 biological replicates). Data represented as geometric mean ± SD.

Article Snippet: Assays with HEK293T pGL4.45 reporter cells were performed as described previously., Briefly, 3×10 5 cells/mL were added to a 96-well plate in 100 μL of DMEM with 10% FBS and P/S, then allowed to incubate for 24 h. To each well, 20 μL of treatment specified in each figure caption was added, then allowed to incubate for 24 h. Luciferase was quantified using the Firefly Luciferase Assay Kit (Biotium).

Techniques: Sequencing, Synthesized, Circular Dichroism, Activity Assay, Derivative Assay, Luciferase, Expressing, Control, Transfection